LAMA PENYIMPANAN SERUM, PLASMA EDTA, PLASMA SITRAT TERHADAP TITER WIDAL PADA TERSANGKA DEMAM TIFOID

Abstrak

Typhoid fever is the most common type of salmonellosis. Another type of enteric fever is paratyphoid fever caused by S. paratyphi A, S. paratyphi B and S. paratyphi C. Typhoid fever show more severe symptoms than other enteric fever. Widal test generally uses serum, because it is not mixed with other substances that can be affected. However, in some clinics and hospitals use plasma samples, because widal test is usually carried out in conjunction with routine hematological test. This study was conducted to determine the effect of storage time of serum, plasma EDTA, and plasma citrate on the titer result of widal test in suspected typhoid fever. The type of research conducted is a quasi-experimental. The sample used was 5 people suspected of typhoid fever. Widal was tested with serum samples, plasma EDTA, and plasma citrate with variations in storage time of 0, 1 and 2 days. Widal test is carried out using the slide method. From the results of the study it was found that the minimum titer results were 80 and maximum 320 used serum and plasma EDTA, whereas for plasma citrate samples the results were a minimum of 80 and a maximum of 160. With the statistical test obtained p. value (sig) 0.008 < sig 0.05, it can be interpreted that there is an effect of the type of sample on widal test.