ANALISIS PERSENTASE LIMFOSIT DAN MONOSIT MENCIT BALB/C SETELAH DIPAPAR MEDAN ELEKTROMAGNETIK EXTREMELY LOW FREQUENCY 500 μT DAN 1000 μT
Abstract
Extremely Low Frequency (ELF) electromagnetic waves are generated by electrical devices and power systems (0 - 300 Hz). The rapid development of technology and the use of electronics cause adverse biological effects on humans. However, the biological effects of exposure to ELF electromagnetic fields are not fully clarified. The purpose of this study was to examine the effect of ELF electromagnetic field exposure on the percentage of lymphocytes and monocytes in Balb/C mice. This type of research was true experimental with a completely randomized design (CRD) design. Balb / C mice used were 18 male Balb / C mice with a body weight of 15 - 20 grams consisting of a control group, 500 µT experimental group, and 1000 µT experimental group. The treatment was given intermittently 3 hours/day for 30 days. Statistical analysis using One Way Anova with LSD (Least Significance Different) and Kruskal Wallis test. The results showed no significant difference in the average percentage of lymphocytes and monocytes of Balb/C mice between the control group, the 500 μT experimental group and the 1000 μT experimental group. Further research needs to be done on the effect of electromagnetic fields with greater intensity and longer exposure time on lymphocytes and monocytes.
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References
2. Kanza NRF, Sudarti S, Maryani M. Pengaruh Paparan Medan Magnet Extremely Low Frequency (ELF) Terhadap pH dan Daya Hantar Listrik Pada Proses Fermentasi Basah Kopi Liberica (Coffea liberica) dengan Penambahan α-Amilase. ORBITA J Kajian, Inov dan Apl Pendidik Fis. 2020;6(2):315. doi:10.31764/orbita.v6i2.3294
3. Sudarti S, Permatasari E, Ningtyias FW, Mina NM, Laksmiari K. Analysis of Vitamin C Resistance in Red Grapes (Vitis vinifera) After Exposure to Extremely Low Frequency (ELF) Magnetic Fields Intensity 700 uT and 900 uT. J Penelit Pendidik IPA. 2022;8(2):620-626. doi:10.29303/jppipa.v8i2.1386
4. World Health Organization. Environmental Health Criteria 238: Extremely Low Frequency Fields. WHO; 2007. https://www.who.int/publications/i/item/9789241572385
5. International Labour Organization. VLF and ELF Electric and Magnetic Fields. Encylopaedia of Occupational Health and Safety. Published 2011. https://www-iloencyclopaedia-org.translate.goog/part-vi-16255/radiation-non-ionizing/item/658-vlf-and-elf-electric-and-magnetic-fields?_x_tr_sl=en&_x_tr_tl=id&_x_tr_hl=id&_x_tr_pto=tc
6. Wei J, Sun J, Xu H, Shi L, Sun L, Zhang J. Effects of extremely low frequency electromagnetic fields on intracellular calcium transients in cardiomyocytes. Electromagn Biol Med. 2015;34(1):77-84. doi:10.3109/15368378.2014.881744
7. Dayem AA, Hossain MK, Lee S Bin, et al. The Role of Reactive Oxygen Species (ROS) in the Biological Activities of Metallic Nanoparticles. Int J Mol Sci. 2017;18(1):1-21. doi:10.3390/ijms18010120
8. Bassoy EY, Walch M, Martinvalet D. Reactive Oxygen Species: Do They Play a Role in Adaptive Immunity? Front Immunol. 2021;12:755856. doi:10.3389/fimmu.2021.755856
9. Hutagalung MS. Panduan Lengkap Stroke: Mencegah, Mengobati Dan Menyembuhkan. Nusamedia; 2019.
10. Khotimah C, Pramyrtha E, M SP, K S, Y LR. Pengaruh Pemberian Propolis terhadap Jumlah dan Hitung Jenis Leukosit Darah Mencit (Mus musculus) Jantan. J Basic Med Vet. 2015;4(1):28-34.
11. Prakoeswa FR. Peranan Sel Limfosit Dalam Imunulogi: Artikel Review. J Sains dan Kesehat. 2020;2(4):525-537. doi:10.25026/jsk.v2i4.212
12. Olingy CE, Dinh HQ, Hedrick CC. Monocyte heterogeneity and functions in cancer. J Leukoc Biol. 2019;106(2):309-322. doi:10.1002/JLB.4RI0818-311R
13. Kleijn S de, Ferwerda G, Wiese M, et al. A short-term extremely low frequency electromagnetic field exposure increases circulating leukocyte numbers and affects HPA-axis signaling in mice. Bioelectromagnetics. 2016;37(7):433-443. doi:10.1002/bem.21998
14. Lai J, Zhang Y, Zhang J, et al. Effects of 100-μT extremely low frequency electromagnetic fields exposure on hematograms and blood chemistry in rats. J Radiat Res. 2016;57(1):16-24. doi:10.1093/jrr/rrv059
15. Luo X, Jia S, Li R, Gao P, Zhang Y. Occupational exposure to 50 Hz magnetic fields does not alter responses of inflammatory genes and activation of splenic lymphocytes in mice. Int J Occup Med Environ Health. 2016;29(2):277-291. doi:10.13075/ijomeh.1896.00519
16. Wyszkowska J, Jędrzejewski T, Piotrowski J, Stankiewicz M, Kozak W. Evaluation of the influence of in vivo exposure to extremely low-frequency magnetic fields on the plasma levels of pro-inflammatory cytokines in rats. Int J Radiat Biol. 2018;94(10):909-917. doi:10.1080/09553002.2018.1503428
17. Masaenah E, Inawati I, Annisa FR. AKTIVITAS EKSTRAK ETANOL BUAH BELIMBING WULUH (Averrhoa bilimbi L) TERHADAP PENURUNAN KADAR GLUKOSA DARAH MENCIT JANTAN (Mus musculus). J Farmamedika (Pharmamedica Journal). 2019;4(2):37-47. doi:10.47219/ath.v4i2.79
18. Nugroho RA. Mengenal Mencit Sebagai Hewan Laboratorium. Mulawarman University Press; 2018.
19. Smith JB, Mangkoewidjojo. Pemeliharaan, Pembiakan, Dan Penggunaan Hewan Percobaan Di Daerah Tropis. Universitas Indonesia; 1988.
20. Wulandari Y, Hartoyo P, Anita F, Purwatiningsih. Analisis Jumlah Kadar Hemoglobin Dan Sel Darah Putih ( Leukosit ) Pada Mencit ( Mus Musculus ) Sebelum Dan Sesudah Radiasi Gamma Co-60 Dengan Berbagai Variasi Dosis. J Ilm GIGA. 2014;17(1):9-18.
21. Ariana INT, Oka AA, Suranjaya IG, Berata IK. Peningkatan Limfosit, Monosit, dan Basofil pada Sapi Bali yang Digembalakan di Tempat Pembuangan Akhir Sampah Kota Denpasar. J Vet. 2018;19(1):109. doi:10.19087/jveteriner.2018.19.1.109
22. Kawiadji AA, Aspriyanto D, Setyawardhana RHD. Dentin Mencit Jantan ( Mus Musculus ) Yang Dipapar Sinar-X Radiografi. J Kedokt Gigi. 2022;VI(1):19-23.
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