Respiratory viruses detection using the FilmArray Panel
Abstract
Background: Respiratory infections, including pneumonia, are a leading cause of global mortality. These infections can be caused by various pathogens, including typical and atypical bacteria, as well as viruses, fungi, and parasites. Rapid and accurate diagnosis is crucial, especially in cases requiring intensive care, to ensure timely and appropriate treatment.
Objective: This research aimed to obtain an overview of the application of the FilmArray method, a nested multiplex pcr base method, in determining the diagnosis and management of respiratory tract infections.
Methods: This study was a cross-sectional study with a descriptive analytic design on patients with respiratory tract infections. The study was conducted from April to June 2022 at the Biomolecular Laboratory, Laboratory Installation of RSPI Prof. Dr. Sulianti Saroso.
Results: Of the 72 patients examined, 27 (37.5%) tested positive for one of the viruses, while 45 (62.5%) tested positive for neither viruses nor bacteria. Among the positive patients, the most commonly detected virus was human rhinovirus/enterovirus (48%), followed by SARS-CoV-2 (48%), and human metapneumovirus (4%). No atypical bacteria or a double infection of virus and bacteria were detected.
Conclusion: This research indicates that molecular-based methods using the FilmArray respiratory panel can be used for the rapid and accurate detection of respiratory tract infection cases, especially those caused by respiratory viruses and atypical bacteria.
Full text article
References
2. Troeger C, Blacker B, Khalil IA, et al. Estimates of the global, regional, and national morbidity, mortality, and aetiologies of lower respiratory infections in 195 countries, 1990–2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet Infect Dis. 2018;18(11):1191-1210. doi:10.1016/S1473-3099(18)30310-4
3. Vos T, Lim SS, Abbafati C, et al. Global burden of 369 diseases and injuries in 204 countries and territories, 1990–2019: a systematic analysis for the Global Burden of Disease Study 2019. The Lancet. 2020;396(10258):1204-1222. doi:10.1016/S0140-6736(20)30925-9
4. Bender RG, Sirota SB, Swetschinski LR, et al. Global, regional, and national incidence and mortality burden of non-COVID-19 lower respiratory infections and aetiologies, 1990–2021: a systematic analysis from the Global Burden of Disease Study 2021. Lancet Infect Dis. 2024;24(9):974-1002. doi:10.1016/S1473-3099(24)00176-2
5. Jamrozik E, Musk AW. Respiratory health issues in the Asia–Pacific region: An overview. Respirology. 2011;16(1):3-12. doi:10.1111/j.1440-1843.2010.01844.x
6. Kementerian Kesehatan RI. Laporan Nasional: Riset Kesehatan Dasar (Riskesdas) 2018. Lembaga Penerbit Badan Penelitian Dan Pengembangan Kesehatan, Jakarta.; 2019.
7. Cillóniz C, Civljak R, Nicolini A, Torres A. Polymicrobial community‐acquired pneumonia: An emerging entity. Respirology. 2016;21(1):65-75. doi:10.1111/resp.12663
8. Cannon GA, Carr MJ, Yandle Z, et al. A low density oligonucleotide microarray for the detection of viral and atypical bacterial respiratory pathogens. J Virol Methods. 2010;163(1):17-24. doi:10.1016/j.jviromet.2009.07.005
9. Rogers BB, Shankar P, Jerris RC, et al. Impact of a Rapid Respiratory Panel Test on Patient Outcomes. Arch Pathol Lab Med. 2015;139(5):636-641. doi:10.5858/arpa.2014-0257-OA
10. Molina FJ, Botero LE, Isaza JP, et al. Diagnostic concordance between BioFire® FilmArray® Pneumonia Panel and culture in patients with COVID-19 pneumonia admitted to intensive care units: the experience of the third wave in eight hospitals in Colombia. Crit Care. 2022;26(1):130. doi:10.1186/s13054-022-04006-z
11. Gosert R, Koller R, Meyer J, et al. Multicenter Evaluation of the QIAstat-Dx and the BioFire Multiplex Panel Tests for the Detection of Respiratory Pathogens. J Med Virol. 2024;96(12). doi:10.1002/jmv.70129
12. Eckbo EJ, Locher K, Caza M, Li L, Lavergne V, Charles M. Evaluation of the BioFire® COVID-19 test and Respiratory Panel 2.1 for rapid identification of SARS-CoV-2 in nasopharyngeal swab samples. Diagn Microbiol Infect Dis. 2021;99(3):115260. doi:10.1016/j.diagmicrobio.2020.115260
13. Kementerian Kesehatan RI. Laporan Survei Kesehatan Indonesia 2023 Dalam Angka.; 2023.
14. Li ZJ, Zhang HY, Ren LL, et al. Etiological and epidemiological features of acute respiratory infections in China. Nat Commun. 2021;12(1). doi:10.1038/s41467-021-25120-6
15. Hong S, Li D, Wei Y, et al. Epidemiology of respiratory pathogens in patients with acute respiratory tract infection in Xiamen, China: A retrospective survey from 2020 to 2022. Heliyon. 2023;9(11). doi:10.1016/j.heliyon.2023.e22302
16. Chen H, Weng H, Lin M, et al. The Clinical Significance of FilmArray Respiratory Panel in Diagnosing Community-Acquired Pneumonia. Biomed Res Int. 2017;2017:1-6. doi:10.1155/2017/7320859
17. Brendish NJ, Malachira AK, Armstrong L, et al. Routine molecular point-of-care testing for respiratory viruses in adults presenting to hospital with acute respiratory illness (ResPOC): a pragmatic, open-label, randomised controlled trial. Lancet Respir Med. 2017;5(5):401-411. doi:10.1016/S2213-2600(17)30120-0
18. Qian Y, Ai J, Wu J, et al. Rapid detection of respiratory organisms with FilmArray respiratory panel and its impact on clinical decisions in Shanghai, China, 2016‐2018. Influenza Other Respir Viruses. 2020;14(2):142-149. doi:10.1111/irv.12701
19. Leber AL, Everhart K, Daly JA, et al. Multicenter Evaluation of BioFire FilmArray Respiratory Panel 2 for Detection of Viruses and Bacteria in Nasopharyngeal Swab Samples. J Clin Microbiol. 2018;56(6). doi:10.1128/JCM.01945-17
20. Berry GJ, Zhen W, Smith E, et al. Multicenter Evaluation of the BioFire Respiratory Panel 2.1 (RP2.1) for Detection of SARS-CoV-2 in Nasopharyngeal Swab Samples. J Clin Microbiol. 2022;60(5). doi:10.1128/jcm.00066-22
21. Kementerian Kesehatan RI. Surveilans ILI-SARI dan COVID-19. available at https://sites.google.com/view/surveilans-ili-sari/dashboard.
22. Calderaro A, Buttrini M, Farina B, Montecchini S, De Conto F, Chezzi C. Respiratory Tract Infections and Laboratory Diagnostic Methods: A Review with A Focus on Syndromic Panel-Based Assays. Microorganisms. 2022;10(9):1856. doi:10.3390/microorganisms10091856
Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.