PEMERIKSAAN GOLONGAN DARAH ABO MENGGUNAKAN HOMEMADE ANTISERA SERUM DAN PLASMA
Abstract
Blood type is determined by the presence of antigens on the surface of the red blood cells. The most commonly used ABO system blood group examination is the slide method using antisera reagents. In general, commercial antisera are used, which are relatively expensive. This study aims to make homemade antisera more affordable from serum and plasma materials and to compare the results with commercial reagents. This research is an experiment with a static group comparison design. There were 55 samples with 12 A blood group categories, 13 B blood types, 5 AB blood groups, and 25 O blood groups. The samples were then examined for ABO blood group using commercial antisera and homemade antisera, then compared to the results of statistical data analysis in research using the Friedman test. The results of the Friedman test analysis showed that the significance value for blood group A > 0.05 was 1.000, the significance value for blood group B > 0.05 was 1.000, the significance value for blood group AB > 0.05 was 1.002, and the significance value for blood group O > 0.05 was 1.000. Based on these statistical results, it can be concluded that there is no significant difference in the results of the ABO system blood group examination using commercial antisera reagents and homemade antisera from serum and plasma A, B, and O.
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