UJI EKSTRAK ETANOL DAUN SRIKAYA (ANNONA SQUAMOSA) TERHADAP DAYA HAMBAT BAKTERI SHIGELLA DYSENTERIAE
Abstract
Srikaya leaves contain chemical compounds of flavonoids, saponins, alkaloids, tannins and phenolics that can be used to inhibit the growth of Shigella dysentriae bacteria that cause diarrhea and dysentery. This study aimed to determine the effectiveness of Srikaya leaf ethanol extract (Annona squamosa) against Shigella dysenteriae bacteria by measuring inhibitory zones with concentrations of 65%, 70%, 75%, and 80% against Shigella dysentriae bacteria on Muler Hinton agar media. The design of the pseudo-experimental research using a descriptive test. This research method was a diffusion method to measure the inhibition zone of shigella dysentriae in MHA Kirby Bauer media. After obtaining the inhibition zone, an analysis of the effectiveness of Srikaya leaf ethanol extract (Annona squamosa) with concentrations of 65%, 70%, 75% and 80% against Shigella dysentriae bacteria on Muler Hinton agar media was carried out. From the results of the study, the mean value of the 65% concentration inhibition zone was 7.91 mm, the concentration of 70% was 9.41 mm, the concentration of 75% was 12.50 mm and the concentration of 80% was 13.25 mm. So that the average inhibitory zones formed at concentrations of 65% and 70% are included in the resistant category, then the average inhibitory zones formed at concentrations of 75% and 80% are included in the intermediate category against Shigella dysentriae bacteria when compared to Tetracycline. The conclusion of this study was that srikaya leaf extract is not effective in inhibiting Shigella dysentriae bacteria, because it does not have the same sensitive category ability as Tetracycline.
Full text article
References
2. Mamitoho R, Suling PL, Mintjelungan C. Uji Daya Hambat Perasan Buah Srikaya (Annona squamosa L) Terhadap Pertumbuhan Candida albicans. J Ilm Farm - UNSRAT. 2018;7(3):9-13. https://ejournal.unsrat.ac.id/index.php/pharmacon/article/view/20100
3. Putra IGIP. Uji Aktivitas Antikanker Ekstrak Etanol Biji Srikaya (Annona squamosa L.) Terhadap Beberapa Sel Kanker Manusia Secara In Vitro. Published online 2015.
4. Agus C, Adriyanti DT, Syahbudin A, Basori AF. Tanaman Langka Indonesia: Di KP4 UGM. UGM PRESS; 2018.
5. Winarti S, Djajati S, Ning Tyas LA. Potensi Bubuk Biji Srikaya Dan Bubuk Daun Jambu Biji Sebagai Pengawet Alami Ikan Wader (Rasbora lateristriata) Selama Penyimpanan Suhu Rendah Potential of Srikaya Seed and Guava Leaf Powder as Natural Preservatives for “Wader” Fish (Rasbora lateristriata). Desember. 2020;14(2):15.
6. Yudhana A, Praja RN. Efektivitas Larvasida Ekstrak Biji Srikaya (Annona squamosa L.) terhadap Mortalitas Larva Anopheles aconitus. veterina Med. 2016;9(3):73-77.
7. Isramilda I, Sahreni S, Saputra AI. Uji Konsentrasi Daya Hambat Rebusan Daun Srikaya (Annona squamosa L.) Terhadap Pertumbuhan Staphylococcus aureus. BEST J (Biology Educ Sains Technol. 2020;3(1):01-08. doi:10.30743/best.v3i1.2378
8. Aryantini D, Sari F, Wijayanti CR. Kandungan Fenolik Dan Flavonoid Total Pada Ekstrak Daun Srikaya (Annona squamosa L.) Terfermentasi. Farmasains J Ilm Ilmu Kefarmasian. 2020;7(2):67-74. doi:10.22236/farmasains.v7i2.5635
9. Timur WW, Wijayanti R, Kamil TA. Uji Aktivitas Ekstrak Daun Srikaya ( Annona squamosa ) sebagai Antipiretik pada Tikus Jantan Galur Wistar secara Invivo Activity Test of Srikaya Leaf Extract ( Annona squamosa ) as an Antipireticin Invivo Wistar Rats. J ad-Dawaa’ J Pharm Sci. 2018;1(1):1-7.
10. Cortes D, Moreno L, Párraga J, Galán A, Cabedo N. Nuevos fármacos inspirados en Annonáceas. Rev Bras Frutic. 2014;36:22-31. doi:10.1590/S0100-29452014000500003
11. Ente ZF, Rumape O, Duengo S. Ekstrak Metanol Daun Srikaya (Annona squamosa L.) Sebagai Insektisida Nabati Terhadap Mortalitas Hama Ulat Grayak (Spodoptera litura). Jambura J Chem. 2020;2(1):1-9. doi:10.34312/jambchem.v2i1.2464
12. Alpha Cindy Elora, Siti Hazar, Sri Peni Fitrianingsih. Kajian Pustaka Efek Farmakologi Daun Srikaya (Annona squamosa L.). Bandung Conf Ser Pharm. 2022;2(2):307-313. doi:10.29313/bcsp.v2i2.4147
13. Kusmardiyani S, Wandasari F, Wirasutisna KR. Telaah Fitokimia Daun Srikaya (Annona squamosa L.) yang Berasal dari Dua Lokasi Tumbuh. Acta Pharm Indones. 2012;37(1):9-13. doi:10.5614/api.v37i1.4030
14. Irawan DD, Rohmah AN, Rahmawati EI, Primindari RS, Rahmawati SA. Antimicrobial Effects of Annona squamosa Leaf Extract on Staphylococcus aureus Growth: An In Vitro Study. J Bidan Cerdas. 2023;5(2):58-65. doi:10.33860/jbc.v5i2.2167
15. Aini F. Isolasi dan Identifikasi Shigella sp. Penyebab Diare pada Balita. Bio-site. 2018;04(1):1-40. https://online-journal.unja.ac.id/BST/article/download/5012/8869/13320
16. Sari N, Erina, Abrar M, Wardani E, Fakhrurrazi, Daud R. Isolasi dan Identifikasi Salmonella Sp dan Shigella Sp Pada Feses Kuda Bendi. J Chem Inf Model. 2018;2(3):401-410.
17. Munfaati PN, Ratnasari E, Trimulyono G. Aktivitas senyawa antibakteri ekstrak herba meniran (Phyllanthus niruri) terhadap pertumbuhan bakteri Shigella dysenteriae secara In Vitro. Lentera Bio. 2015;4(1):64-71.
18. Rakasiwi D, Astuti W, Marliana E. Potensi Antibakteri Ekstrak Etanol Meniran (Phyllanthus niruri L.) Terhadap Bakteri Streptococcus sobrinus dan Salmonella typhi . J At. 2023;08(1):23-27.
19. Ginting NB, Ginting G, Silalahi N. Sistem Pakar Mendiagnosa Penyakit Sampar Menggunakan Metode Hybrid Case Based. J Media Inform Budidarma. 2019;3(1):65. doi:10.30865/mib.v3i1.1062
20. Tarigan P, Siregar SR. Sistem Pakar Mendiagnosa Penyakit Disentri Menerapkan Metode Hybrid Case Based. Maj Ilm Inti. 2018;13(Mei):152-156.
21. Eny W. Uji Daya Hambat Air Rebusan Daun Tapak Dara (Catharanthus roseus (L.) G. Don) terhadap pertumbuhan Shigella dysenteriae. Published online 2020.
22. Utami YP, Umar AH, Syahruni R, Kadullah I. Standardisasi Simplisia dan Ekstrak Etanol Daun Leilem ( Clerodendrum. J Pharm Med Sci. 2017;2(1):32-39.
23. Najib A, Malik A, Ahmad AR, Handayani V, Syarif RA, Waris R. Standardisasi Ekstrak Air Daun Jati Belanda dan Daun Jati Hijau. J Fitofarmaka Indones. 2018;4(2):241-245.
24. Utami YP. Pengukuran Parameter Simplisia Dan Ekstrak Etanol Daun Patikala (Etlingera elatior (Jack) R.M. Sm) Asal Kabupaten Enrekang Sulawesi Selatan. Maj Farm dan Farmakol. 2020;24(1):6-10. doi:10.20956/mff.v24i1.9831
25. Luliana S, Purwanti NU, Manihuruk KN. Pengaruh Cara Pengeringan Simplisia Daun Senggani (Melastoma malabathricum L.) Terhadap Aktivitas Antioksidan Menggunakan Metode DPPH (2,2-difenil-1-pikrilhidrazil). Pharm Sci Res. 2016;3(3):120-129. doi:10.7454/psr.v3i3.3291
26. Anggita D, Nurisyah S, Wiriansya EP. Mekanisme Kerja Antibiotik: Review Article. UMI Med J. 2022;7(1):46-58. doi:10.33096/umj.v7i1.149
27. Agustin N, Shalsabilla SE, Syahidah RN. Jurnal DIFUSI, OSMOSIS DAN IMBIBISI, Fisiologi Tumbuhan. Difusi, Osmosis Dan Imbibisi. Published online 2020:1-6.
28. Adriana Y. Uji Daya Hambat Ekstrak Daun Sirih Hijau (Piper betle Linn) terhadap Bakteri Staphylococcus aureus. 2023;2(2):163-172.
29. Tansil AYM, Nangoy E, Posangi J, Bara RA. Uji daya hambat ekstrak etanol daun srikaya ( Annona squamosa ) terhadap pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus Kandidat Skripsi Fakultas Kedokteran Universitas Sam Ratulangi Manado oleh masyarakat Alor Utara di Nusa Tenggara Timur. J e-Biomedik. 2016;4(2):37-46.
30. Novianti D. Toksisitas Ekstrak Daun Srikaya (Annona squamosa Linn.) Terhadap Jamur Fusarium sp. Sainmatika J Ilm Mat dan Ilmu Pengetah Alam. 2019;16(2):130. doi:10.31851/sainmatika.v16i2.3247
31. Rianto L, Handayani IA, Septiyani A. Uji Aktivitas Ekstrak Etanol 96% Biji Srikaya (Annona squamosa L.) Sebagai Antidiare Yang Disebabkan oleh BakterI Shigella dysenteriae dengan Metode Difusi Cakram. J Ilm Manuntung. 2017;1(2):181-186. doi:10.51352/jim.v1i2.33
Authors
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.