DIFFERENCES IN THE STABILITY OF CHOLINESTERASE ENZYME ACTIVITY OF SERUM AND HEPARIN PLASMA SAMPLES USING PHOTOMETRIC KINETIC METHOD
Abstrak
Errors in laboratory testing can occur at any phase of the testing process, but most errors occur in the pre-analytical phase with a contribution of 70%. Cholinesterase is an examination in pesticide poisoning to detect chronic and even deadly health problems. This study aimed to determine the stability of cholinesterase enzyme activity in serum and plasma heparin samples. This research was conducted at the Clinical Chemistry Laboratory of Bandung Polytechnic Medical Laboratory Technology in May 2024. This study was quasi-were both of serum and plasma heparin samples stored for 6 and 24 hours ... at room temperature and then the levels were compared were immediately. The examination was carried out by the photometric kinetic method using a photometer with a wavelength of 405 nm and replicated 3 times then the data processed using SPSS with the general linear model (GLM) test. Statistically, the GLM test results on serum and plasma heparin samples showed a sig value. 0.000 where the sig value. <0.05, so there is a difference in stability between serum and plasma heparin. However, based on the results of clinical analysis, the Total Error (TE%) value with a delay of 6 hours and 24 hours for immediate examination in serum samples was 1.68% and 6.44%, respectively. While in heparin plasma samples it was 3.66% and 6.95%. Cholinesterase examination had a TEa% value of 8.9%. So the conclusion both serum and heparin plasma samples have TE% < TEa% values, based on these results there is no clinical difference.
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2. Ngetich EK, Kiprono S, Marera D. Stability of Selected Biochemical Analytes in Serum Stored at Room Temperature for 24 Hours at Kericho County Referral Hospital, Kenya. East African J Heal Sci. 2022;5(1):300-310. doi:10.37284/eajhs.5.1.844
3. Santi OD, Rosita L, Cahyaningrum YD. Pengaruh Suhu dan Interval Waktu Penyimpanan Sampel Serum pada Pengukuran Kadar Glukosa Darah. JKKI J Kedokt dan Kesehat Indones. 2011;3(8):39-43.
4. Tutu CG, Manampiring AE, Umboh A. Faktor-faktor yang Berhubungan dengan Aktivitas Enzim Cholinesterase Darah pada Petani Penyemprot Pertisida. Indones J Public Heal Community Med. 2020;1(4):40-53.
5. WHO. Environmental Health Criteria 63. Organophosporus insecticides: a general introduction. :1-82.
6. Mukhametgalieva AR, Zueva I V., Aglyamova AR, Lushchekina S V., Masson P. A new sensitive spectrofluorimetric method for measurement of activity and kinetic study of cholinesterases. Biochim Biophys Acta - Proteins Proteomics. 2020;1868(1):140270. doi:10.1016/j.bbapap.2019.140270
7. Djuric V, Mitic V, Deletic N, Ilic M, Cirkovic B, Stankov-Jovanovic V. A novel kinetic cholinesterase-inhibition based method for quantification of biperiden in pharmaceutical preparations. Hem Ind. 2020;74(4):265-271. doi:10.2298/hemind200413021d
8. Zivkovic AR, Decker SO, Zirnstein AC, et al. A sustained reduction in serum cholinesterase enzyme activity predicts patient outcome following sepsis. Mediators Inflamm. 2018;2018. doi:10.1155/2018/1942193
9. Tunsaringkarn T, Zapuang K, Rungsiyothin A. The correlative study of serum pseudo-cholinesterase, biological parameters and symptoms among occupational workers. Indian J Clin Biochem. 2013;28(4):396-402. doi:10.1007/s12291-013-0322-3
10. Araoud M, Mhenni H, Hellara I, et al. Étude De L’Activité De La Cholinestérase Plasmatique Au Cours Des Atteintes Hépatiques. Ann Biol Clin (Paris). 2013;71(3):293-298. doi:10.1684/abc.2013.0819
11. Kazmierczak, Steven Azzazy HME. Diagnostic Enzymology. Berlin, Bost Gruyter. Published online 2014. doi:https://doi.org/10.1515/9783110227802le
12. Chindara CAN, Kurnaeni N, Nurhayati B, Rinaldi Feisal S. Stabilitas Aktivitas ALT Serum, Plasma Heparin dan Plasma EDTA pada Suhu Simpan 2 - 8°C. J Ris Kesehat Poltekkes Depkes Bandung. 2019;11(1):299-305.
13. Wei YH, Zhang CB, Yang XW, Ji M De. The feasibility of using lithium-heparin plasma from a gel separator tube as a substitute for serum in clinical biochemical tests. Lab Med. 2010;41(4):215-219. doi:10.1309/LMIXVAI70KS0UWQI
14. Blazheyevskiy MY, Koval’ska O V., Diadchenko V V. The determination of the cholinesterase activity using 3,3’,5,5’-tetramethylbenzidine as an Indicator. 2021;19(75):3-8.
15. Bisswanger H. Enzyme assays. Perspect Sci. 2014;1(1-6):41-55. doi:10.1016/j.pisc.2014.02.005
16. Atmanto KAA, Mutmainnah M, Samad IA. Cholinesterase (ChE) Test Using Ellman’s Photometric Method. Int J Sci Adv. 2022;3(3):475-478. doi:10.51542/ijscia.v3i3.29
17. Masson P. Time-dependent kinetic complexities in cholinesterase-catalyzed reactions. Biochem. 2012;77(10):1147-1161. doi:10.1134/S0006297912100070
18. Alias AS. Electrometric method determination of blood cholinesterase activity in stray dogs. Iraqi J Vet Sci. 2014;28(2):153-159. doi:10.33899/ijvs.2014.116934
19. Moorhead KA, Discipulo ML, Hu J, Moorhead RC, Johns JL. Alterations due to dilution and anticoagulant effects in hematologic analysis of rodent blood samples on the Sysmex XT-2000iV. Vet Clin Pathol. 2016;45(2):215-224. doi:10.1111/vcp.12338
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