Interpretation of staining results using sappan wood (Caesalpinia sappan l.) as an eosin substitute in hematoxylin eosin staining
Abstract
Background: An important stage in histopathological examination is the tissue staining process where the staining technique uses Hematoxylin Eosin. Eosin as a dye component is carsinogenic and poses a risk to health and the environment if used in the long term. Sappan wood containing brazilin has the potential to be developed as a natural alternative to eosin
Objective: This study aims to determine the interpretation of staining results using sappan wood as a substitute for eosin in hematoxylin eosin staining
Methods: This study is an experimental study with an intact Group Comparoson design consisting of two treatment groups, namely staining using eosin and sappan wood. Data analysis was carried out using the Chi square test. This study was conducted at the Barokah Palembang anatomical pathology laboratory on March 16-17, 2025.The population taken was histological tissue preparations in this study werw 32 sampels calculated using the Federer formula.
Results: staining using eosin with 100% very good quality of preparation, for staining using sappan wood the quality of preparation is not good 6.3%, less good 50% and good quality 43.8%. The results of the statistical test obtained p = 0.000 < 0.05 this indicates a significant difference between the results of staining using eosin and sappan wood filtrate
Conclusion: Sappan wood staining has the potential as a natural dye to replace eosin in Hematoxylin-Eosin staining.
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