PERBANDINGAN HASIL PEMERIKSAAN MIKROSKOPIS BASIL TAHAN ASAM METODE DEKONTAMINASI DENGAN METODE TES CEPAT MOLEKULER
Abstract
Tuberculosis (TB) remains a serious public health problem because of its high potential for person-to-person transmission. Sputum examination using NaOH 4% has a higher sensitivity compared to direct acid-fast basilli smear that has a sensitivity 64.8% and 30%, respectively. New methods have been widwly developed for rapid detection and identification of Mycobacterium tuberculosis (MTB) in active tuberculosis with the best techniques such as genomic or molecular examination. An automated molecular testing for Mycobacterium tuberculosis identification has a similar sensitivity with culture as gold standard method. The aim of this study to compare the results between microscopic examination of acid-fast bacilli decontamination method and molecular examination. This study is a comparative study with cross sectional approach which held in Microbiology Laboratory of Center of Lung Health of Makassar. The results of the acid-fast bacilli decontamination and the molecular method have diagnostic result statisticaly analysis showed no significant difference.
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References
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Swarjana, I. K. (2014). Metodologi Penelitian Kesehatan. Denpasar: Andi.
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Umar, B. (2007). Analisis Hasil Pemeriksaan Miksrokopik Basil Tahan Asam Mycobacterium Tuberculosis Penderita TB Paru Puskesmas Rujukan Mikroskopis Somba Opu Kabupaten Gowa.
World Health Organization. (2016). Tuberculosis. Retrieved Desember 14, 2017, from www.who.int/tb/...report/gtbr2016_executive_summary.pdf
Alsagaff, H., & Mukty, H. A. (2010). Dasar-dasar Ilmu Penyakit Paru. Surabaya: Airlangga University Press.
Amran, P., Mursalim, Hadijah, S., & Hasnawati. (2015). Bakteriologi Terapan 3. Makassar: Kementerian Kesehatan RI Politeknik Kesehatan Kemenkes Makassar.
Association of Public Health Laboratories. (2013). Laboratory Considerations for Use of Cepheid Xpert MTB/RIF Assay. Retrieved Juni 25, 2018, from . www.aphl.org/AboutAPHL/publications/Documents/ID_2013Nov_ Cepheid-Xpert-Fact-Sheet.pdf.
Bahtiar, Z. (2017). Perbandingan Hasil Pemeriksaan Basil Tahan Asam Menggunakan Mikroskopis Langsung (Direct) Dengan Metode Kubica (Indirect). Retrieved Desember 14, 2017, from http://zulfitriani28.blogspot.co.id/2017/03/karya-tulis-ilmiah-perbandingan-hasil.html
Brooks, G. F., Carrol, K. C., Butel, J. S., Morse, S. A., & Mietzner, T. A. (2014). Jawetz, Melnick, & Adelberg Mikrobiologi Kedokteran. Jakarta: Penerbit Buku Kedokteran ECG.
CDC (Central of Disease Control). (2009) Updated Guildelines for the Use of Nucleic Acid Amplification Tests in the Diagnosis of Tuberculosis. MMWR. Retrieved Juni 25, 2018, from www.cdc.gov/mmwr/preview/mmwrhtml/mm5801a3. htm?scid=mm5801a3_e.
Frida, E., S, I., & Hardjoeno. (2006). Analisis Temuan BTA pada Sputum Cara Langsung dan Sediaan Konsentrasi Pada Suspek TB. Retrieved Juni 25, 2018 from journal.unair.ac.id/download-fullpapers-IJCPML-12-2-05.pdf.
Gampa, E. S. (2017). Analisis Hubungan Gradasi Basil Tahan Asam Dengan Indeks Eritrosit Pada Penderita Tuberculosis Diagnosis Awal Di BBKPM Makassar.
Kementerian Kesehatan RI. (2012). Standar Prosedur Operasional Pemeriksaan Mikroskopis TB. Retrieved Desember 14, 2017, from www.depkes.go.id/download.php?file=download/.../SopTB/SopTB-2012-TB.pdf
Kementerian Kesehatan RI. (2016). Info Data Tuberkulosis Indonesia. Retrieved Desember 14, 2017, from www.depkes.go.id/download.php?file=download/.../infodatin/InfoDatin-2016-TB.pdf
Kurniawan, E., Raveinal, Fauzar, & Arsyad, Z. (2016). Nilai Diagnostik Metode Real Tme PCR GeneXpert Pada Tuberkulosis Paru BTA Negatif. Retrieved Desember 12, 2017, from jurnal.fk.unand.ac.id/index.php/jka/article/download/609/495
Laboratorium Medik. (2010). GeneXpert MTB/RIF (Pengoperasian Alat, Prosedur, dan Interpretasi Hasil) Untuk Mendiagnosa TBC. Retrieved Desember 17 , 2017, from http://www.infolabmed.com/2017/03/genexpert-mtbrif-pengoperasian-alat.html
Lestari, E. P. (2008). Teknik Sentrifugasi Untuk Meningkatkan Penemuan Basil Tahan Asam Dari Sputum Suspek Tuburkulosis. Retrieved Desember 14, 2017, from repository.unair.ac.id/24163/2/gdlhub-gdl-s1-2010-lestarieka-11462-fkm155-t.pdf
Lynda, A. (2012). Rapid TB Test. Jurnal Tuberkulosis Indonesia. Retrieved Desember 12, 2017, from ppti.info/ArsipPPTI/PPTI-Jurnal-Maret-2012.pdf
Riset Kesehatan Dasar (2013). Departemen Kesehatan. Retrieved Desember 12, 2017, from www.depkes.go.id/resources/download/general/Hasil%20Riskesdas%202013.pdf
Saptawati, L., Mardiastuti, Kurniawati, A., & Rumende, C. M. (2012). Evaluasi Metode FastPlaque Tuberkulosis Untuk Mendeteksi Mycobacterium Tuberculosis Pada Sputum Di Beberapa Unit Pelayanan Kesehatan Di Jakarta . Jurnal Tuberkulosis Indonesia. Retrieved from ppti.info/ArsipPPTI/PPTI-Jurnal-Maret-2012.pdf
Setiati, S., Alwi, I., Sudoyo, A. W., K, M. S., Setiyohadi, B., & Syam, A. F. (2014). Buku Ajar Ilmu Penyakit Dalam. Jakarta: InternaPublishing.
Sirait, N., Parwati, I., Dewi, N. S., & Suraya, N. (2013). Validitas Metode PCR GeneXpert MTB/RIF Pada Bahan Pemeriksaan Sputum Untuk Mendiagnosis Multidrug Resistance Tuberculosis. Retrieved Desember 18, 2017, from download.portalgaruda.org/article.php?...Validitas%20Metode%20Polymerase%20Ch...
Susanty, E. (2015). Uji Diagnostik GeneXpert MTB/RIF Di Rumah Sakit Umum Pusat Haji Adam Malik Medan. Retrieved Desember 16, 2017, from http://repository.usu.ac.id/handle/123456789/49354
Swarjana, I. K. (2014). Metodologi Penelitian Kesehatan. Denpasar: Andi.
Syahrurachman, A., Chatim, A., W.K, A. S., Kurniawati, A., Santoso, A. S., Harun, B. H., . . . Soemarsono, F. (2009). Buku Ajar Mikrobiologi Kedokteran . Jakarta: Binarupa Aksara.
Trisnawati, D. (2016). Pengaruh Kecepatan Sentrifugasi Terhadap Pembacaan Mikroskopis Basil Tahan Asam Pada Pasien Tuberkulosis Dengan Hasil Scanty. Retrieved Desember 14, 2017, from repository.unimus.ac.id/118/1/SKRIPSI%20FULTEXS.pdf
Umar, B. (2007). Analisis Hasil Pemeriksaan Miksrokopik Basil Tahan Asam Mycobacterium Tuberculosis Penderita TB Paru Puskesmas Rujukan Mikroskopis Somba Opu Kabupaten Gowa.
World Health Organization. (2016). Tuberculosis. Retrieved Desember 14, 2017, from www.who.int/tb/...report/gtbr2016_executive_summary.pdf
Authors
Husna, N., & Dewi, N. U. (2020). PERBANDINGAN HASIL PEMERIKSAAN MIKROSKOPIS BASIL TAHAN ASAM METODE DEKONTAMINASI DENGAN METODE TES CEPAT MOLEKULER. JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, 12(2), 316-323. https://doi.org/10.34011/juriskesbdg.v12i2.894
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