AKTIVITAS LARVASIDA JAMUR Metarhizium anisopliae TERHADAP LARVA Aedes aegypti DI LABORATORIUM DAN UJI COBA LAPANGAN

Abstract

The use of synthetic (chemical) insecticides in the control of mosquito vectors raises several problems including causing harm to humans (poisoning), resistance to insects and the occurrence of environmental pollution. The way to reduce the loss of these negative impacts is by developing natural and biological insecticides. The advantage of using natural insecticides is that they do not pollute the environment and are relatively safe for humans. One of the biological insecticides used is the fungus Metarhizium anisopliae which has the potential to control insects in the larval stage. This study was conducted to determine the effectiveness of the fungus Metarhizium anisopliae against Aedes aegypti larvae compared to the standard larvicide Temephos in the laboratory and in the field. Samples eggs of Aedes aegypti obtained from the P2B2 Ciamis Research and Development Workshop were then cultured into third instar larvae. Metarhizium anisopliae fungus strains were obtained from the Ujung Berung Plant and Plantation Protection Agency (BPTP), made in various concentrations through a preliminary test. Field trials were carried out on a small scale by placing mosquito breeding sites (ovitrap). Larvicidal activity was measured by the number of The larva Lethal Concentration 50 (LC50). The results showed that the larvicidal activity of the fungus Metarhizium anisopliae with 50% larval mortality (LC50) in the laboratory and field trials were at a concentration of 10^-1 with 109,200 spores in the laboratory and 81,750 spores in the field. The results of this study that Metarhizium anisopliae may be used as an alternative insecticide for controlling dengue vectors.