PENGARUH VARIASI WAKTU DAN SUHU INKUBASI METIL SALISILAT SEBAGAI CLEARING AGENT TERHADAP KUALITAS PREPARAT JARINGAN LIPOSARKOMA

Abstract

Liposarcoma is a malignant neoplasm with adipocyte differentiation. One subtype of liposarcoma is the atypical lipomatous tumor which is macroscopic in size, while microscopically very similar to normal fat cells. Tissues that contain fat have properties that are difficult to penetrate by the fixative fluid in the process of maturation of tissues to allow for imperfect tissue processing. One of the stages of network maturation that affects the quality of preparations is clearing. Xylol is a commonly used clearing agent. Because of its dangerous and toxic nature, other clearing agents are used. Methyl salicylate is used as a clearing agent because it is non-polar that can dissolve adipose. In addition, methyl salicylates can also dissolve with dehydrating agents and can dissolve with paraffin so that they can harden tissue. The purpose of this study was to find out if there was an influence on time variations and Methyl salicylate incubation temperature as a clearing agent and know the difference with xylol using quasi-experimental methods. The sample uses micecco tissue organs that have been induced with liposarcoma tumor cells in more than 75 preparations from 5 blocks. The parameters used are interpretation quality of core and cytoplasmic color intensity preparations using an imageJ software and clarity of cell membranes by a pathologist. The results showed that there was an effect on the timing and incubation temperature of methyl salicylates on the quality of liposarcoma tissue slides and there wanoot a difference in nucleus color intensity and membranes cell compared to xylol. Research advice is to find the optimal time and temperature of methyl salicylate perfection as a clearing agent to get a good quality slide.