PENGARUH DISINFEKTAN TERHADAP PERTUMBUHAN Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, dan Bacillus subtilis ATCC 6633
Abstract
Contamination of microorganisms, whether bacteria, yeast, mold, or viruses, is a serious problem in microbiology laboratories and in health care facilities. Environmental monitoring (EM) can be used to control contaminating bacteria in the laboratory, the main objective of which is to ensure that the number of airborne particles is within specified limits and regularly estimate the effectiveness of the cleaning and disinfection processes. The correct use of disinfectants and the proper method of use are basic elements in infection prevention in health care. The effectiveness of disinfection procedures depends on many factors (e.g.contact time, final concentration of active ingredient). This research aimed to determine the effect of disinfectants on the growth of Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 9027, and Bacillus subtilis ATCC 6633. This research type was Quasi Experiment. Samples of 70% Alcohol, 70% Isopropyl Alcohol, and 0.75% Benzalkonium chloride were exposed to S. aureus ATCC 6538, P. aeruginosa ATCC 9027, and B. subtilis ATCC 6633 with contact times of 1, 5, and 10 minutes. Statistical data analysis in the study used the Cochran Q test. The research method used Hard Surface Carrier Test. The results of the Cochran Q test analysis showed that the Asymptotic Significance value > 0.05. Based on these statistical results, it can be concluded that there is no significant difference between contact time (1,5 and 10 minutes) and disinfectant (Alcohol 70%, Isopropanol 70%, Benzalkonium chloride 0.75%) on the growth of S. aureus ATCC 6538, P. aeruginosa ATCC 9027, and B. subtilis ATCC 6633.
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