PERBANDINGAN NILAI PENGUKURAN KUANTITATIF HASIL EKSTRAKSI DNA SALMONELLA TYPHI MENGGUNAKAN METODE BOILING, NAOH, KIT KOMERSIAL

Abstrak

Typhoid fever is a disease that often occurs in developing countries, globally S. typhi is the main cause. In Indonesia, cases of typhoid tend to increase from year to year with an average illness of 500 / 100,000 people with deaths between 0.6 - 5%. Laboratory tests to detect typhoid fever are bacteriological based examinations, serological tests, and molecular biology based examinations. DNA extraction is an important step in molecular techniques to obtain DNA isolates.The type of research conducted was quasi-experimental by extracting DNA S.typhi using several methods and variations. The extracted product was measured by its concentration and purity using a Spectrophotometer NanoDrop. The results showed that the highest value was obtained from the extracted product using the commercial kit method, the concentration obtained was 2.15 ng / μL and the purity reached 1.83. In the boiling method, the highest concentration and purity was obtained from extraction by heating 100^oC for 25 minutes, with a concentration of 1.9 ng / μL and purity reaching 1.73. Whereas in the extraction method using NaOH, the highest purity was obtained from extraction using 25 µM NaOH, with a concentration of 1.7 ng / μL and purity reaching 1.72.