EFEKTIVITAS BROMELIN SEBAGAI DEPROTEINASI KUTIKULA PINJAL PADA PROSES PREPARAT PERMANEN

Abstract

Chitin is one of the constituent components of the arthropod exoskeleton. Peptide bonds in chitin can be broken using KOH solution as deproteination. The deproteination process will cause the chitin layer of the exoskeleton to thin. The Bromelin enzyme from pineapple is able to break down peptide bonds. Bromelain will be used in the deproteinase process of the flea chitin exoskeleton. The aim of the research was to determine the ability of bromelain to deproteinize the flea exoskeleton. Quasi-experimental research method, 96 cat fleas. Three treatment groups of 15%, 20% and 25% concentration of bromelain and one control group of 10% KOH solution will be contacted with the fleas, with a soaking time of 24 hours and 48 hours, and carried out three repetition times. The thinning process will end with the application of entelan as an adhesive for the preparation. This research was conducted from April to October 2020, at the Integrated Laboratory and Parasitology Laboratory of the Health Analyst Department, Bandung Health Polytechnic. The results of microscopic examination of the preparations were then analyzed using the Kruskal-Wallis test and the Mann-Whitney test. The results obtained were <0.05 indicating that there was a difference which was significant for all treatments. The results of data analysis produced the highest quality of deproteination in the 25% Bromelin treatment and for the optimal time which produced the highest quality of deproteination at 48 hours.