ISOLASI BROMELIN DARI BUAH NANAS (Ananas comosus L. Merr) DENGAN GARAM DAPUR
Abstract
ABSTRACT
Pineapple fruit is widely known by the people of Indonesia because many plants grow in Indonesia, which has a tropical climate. Pineapple fruit is often used as a meat tenderizer because pineapple contains a bromelain component, which is a sulfhydryl protease enzyme (-SH) which can hydrolyze peptide bonds in proteins into simpler amino acid compound bonds. The process carried out to obtain bromelain from pineapple is an isolation process. Isolation of enzymes can be carried out based on differences in their chemical-physical properties, such as solubility, size, charge, and adsorption characteristics. Isolation of enzymes based on solubility, one of which is by precipitation with inorganic salts. The addition of inorganic salts to the solution containing the enzymes causes the solubility of the enzymes in the solution to decrease, and the enzymes will precipitate. This study aims to isolate bromelain from pineapple with 40% table salt with a pH variation of 6.0; 7.0; 8.0; and 9.0. Done in three repetitions. Determined the optimal pH for isolation, yield value, and specific activity. Analyzed by Anova test. The results showed that the optimal pH for isolating the bromelain enzyme was at pH 9 with a yield of 7.7768%, and the most optimal specific enzyme activity at pH 7 with a value of 2,69 U / mg.
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References
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2. Secor Jr, E. R., Carson VI, W. F., Cloutier, M. M., Guernsey, L. A., Schramm, C. M., WuC. A. & Thrall, R. S. 2005. Bromelain Exerts Anti-Inflammatory Effects in An Ovalbumininduced Murine Model of Allergic Airway Disease. Cell Immunol., 237 (1): 68-75.
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7. Masri, M. 2014. Variasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Bonggol Nanas pada Variasi Suhu & pH. Biogenesis Jurnal Ilmiah Biologi. Vol. 2 No. 2, hal 119-125.
8. Dewi Nurhayati, Firman Solihat,M, Sundara Mulia, Y, Wibowo and Syamsuar. 2018. The Effect Amonium Sulphate Concentration on The Isolation Result of Bromelin Skin and Tubers of Fineapples. International Journal of Current Research
9. Gautam SS, Mishra S, Dash V, Amit K and Rath G. 2010. Cooperative Study or Extraction, Purification and Estimation of Bromelain from Stem and Fruit of Pineapple Plant. Thai J. Pharm., Sci. Vol 1(1):2.
10. Sebayang, F. 2006. Pengujian stabilitas enzim bromelain yang diisolasi dari bonggol nanas serta imobilisasi menggunakan kappa karagenan. Jurnal Sains Kimia. 10:20- 26.
11. Mantell, S.H., Matthew, J.A., and McKee, R.A., 1985, Principles of plant Biotechnology : An in-troduction To Genetic Engineering In Plants, Blackweell Scien Publication: London, p. 120.
DAFTAR PUSTAKA
1. Lehninger. 2008. Principles Of Biochemistry. 5th Ed. H. Freeman and Company New York.
2. Secor Jr, E. R., Carson VI, W. F., Cloutier, M. M., Guernsey, L. A., Schramm, C. M., WuC. A. & Thrall, R. S. 2005. Bromelain Exerts Anti-Inflammatory Effects in An Ovalbumininduced Murine Model of Allergic Airway Disease. Cell Immunol., 237 (1): 68-75.
3. Fileti, A.M.F; Fischer, G.A; Santana, J.C.C; Tambourgi, E.B.2009. Batch and Continuous Extraction of Bromelain Enzyme by Reversed Micelles. Brazilian Archives of Biology and Technology
4. Winarno, F.G., 2002. Kimia Pangan dan Gizi, PT Gramedia Pustaka Utama, Jakarta
5. Subiyantoro. S, 2001, “Mengenal Lebih Jauh Tentang Garam”, BPPP Banyuwangi, Jatim
6. Kumaunang, Maureen, Vanda Kamu. 2011. Aktifitas Enzim Bromelin dari Ekstrak Kulit Nanas (Ananas comosus). FMIPA Universitas Samratulangi Manado. Jurnal Ilmiah SAINS Vol. 11 No. 2, Hlm. 198-201.
7. Masri, M. 2014. Variasi dan Pengukuran Aktivitas Enzim Bromelin dari Ekstrak Kasar Bonggol Nanas pada Variasi Suhu & pH. Biogenesis Jurnal Ilmiah Biologi. Vol. 2 No. 2, hal 119-125.
8. Dewi Nurhayati, Firman Solihat,M, Sundara Mulia, Y, Wibowo and Syamsuar. 2018. The Effect Amonium Sulphate Concentration on The Isolation Result of Bromelin Skin and Tubers of Fineapples. International Journal of Current Research
9. Gautam SS, Mishra S, Dash V, Amit K and Rath G. 2010. Cooperative Study or Extraction, Purification and Estimation of Bromelain from Stem and Fruit of Pineapple Plant. Thai J. Pharm., Sci. Vol 1(1):2.
10. Sebayang, F. 2006. Pengujian stabilitas enzim bromelain yang diisolasi dari bonggol nanas serta imobilisasi menggunakan kappa karagenan. Jurnal Sains Kimia. 10:20- 26.
11. Mantell, S.H., Matthew, J.A., and McKee, R.A., 1985, Principles of plant Biotechnology : An in-troduction To Genetic Engineering In Plants, Blackweell Scien Publication: London, p. 120.
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